Modern experimental biochemistry /
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| Format: | Livre |
| Langue: | anglais |
| Publié: |
San Francisco :
Benjamin Cummings,
c2000. [reprinted]
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| Édition: | 3rd ed. |
| Sujets: | |
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Table des matières:
- Pt. I. Theory and Experimental Techniques
- Ch. 1. Introduction to the Biochemistry Laboratory
- A. Safety in the Laboratory
- B. The Laboratory Notebook
- C. Cleaning Laboratory Glassware
- D. Quantitative Transfer of Liquids
- E. Preparation and Storage of Solutions
- F. Statistical Analysis of Experimental Measurements
- Ch. 2. General Laboratory Procedures
- A. pH Measurements, Buffers, and Electrodes
- B. Dialysis and Ultrafiltration
- C. Lyophilization (Freeze-Drying)
- D. Measurement of Protein Solutions
- E. Measurement of Nucleic Acid Solutions by Spectrophotometry
- Ch. 3. Separation and Purification of Biomolecules by Chromatography
- A. Introduction to Chromatography
- B. Paper and Thin-Layer Chromatography
- C. Gas Chromatography
- D. Column Chromatography (GC)
- E. Ion-Exchange Chromatography
- F. Gel Exclusion Chromatography
- G. High-Performance Liquid Chromatography (HPLC)
- H. Affinity Chromatography and Immunoadsorption
- I. Perfusion Chromatography
- Ch. 4. Characterization of Proteins and Nucleic Acids by Electrophoresis
- A. Theory of Electrophoresis
- B. Methods of Electrophoresis
- C. Practical Aspects of Electrophoresis
- Ch. 5. Spectroscopic Analysis of Biomolecules
- A. Ultraviolet-Visible Absorption Spectrophotometry
- B. Fluorescence Spectrophotometry
- Ch. 6. Radioisotopes in Biochemical Research
- A. Origin and Properties of Radioactivity
- B. Detection and Measurement of Radioactivity
- C. Safety Rules for Handling Radioactive Materials
- Ch. 7. Centrifugation of Biomolecules
- A. Basic Principles of Centrifugation
- B. Instrumentation for Centrifugation
- C. Applications of Centrifugation
- Pt. II. Experiments
- Experiment 1 Using the Biochemical Literature
- Experiment 2 Analytical Methods for Amino Acid Separation and Identification
- Experiment 3 Purification and Characterization of [alpha]-Lactalbumin, a Milk Protein
- Experiment 4 Structural Analysis of a Dipeptide or Tripeptide
- Experiment 5 Protein-Ligand Interactions: Binding of Coomassie Blue Dye to Ovalbumin
- Experiment 6 Kinetic Analysis of an Enzyme, Tyrosinase
- Experiment 7 Purification and Characterization of a Lipid Extracted from Nutmeg
- Experiment 8 Fatty Acid Content of Triacylglycerols in Natural Oils
- Experiment 9 Identification of Carbohydrates by Polarimetry
- Experiment 10 Characterization of Hemoglobin A[subscript 1c] by Affinity Chromatography
- Experiment 11 Isolation, Separation and Spectrophotometric Characterization of Photosynthetic Pigments
- Experiment 12 Photoinduced Proton Transport Through Chloroplast Membranes
- Experiment 13 Isolation, Subfractionation and Enzymatic Analysis of Beef Heart Mitochondria
- Experiment 14 Cholesterol (Total and HDL) and Uric Acid Content of Blood Serum: Enzymes as Reagents in Clinical Chemistry
- Experiment 15 Vitamin C Content of Fruits, Vegetables and Other Foods
- Experiment 16 Activity and Thermal Stability of Acrylamide Gel-Immobilized Peroxidase: An Experiment in Biotechnology
- Experiment 17 Extraction of Chromosomal DNA from Bacterial Cells
- Experiment 18 Characterization of Nucleic Acids by Fluorescence of Bound Ethidium Bromide
- Experiment 19 Preparation, Isolation, and Purification of Bacterial Plasmid DNA
- Experiment 20 Rapid, Microscale Isolation of Plasmid DNA
- Experiment 21 The Action of Restriction Endonucleases on Plasmid or Viral DNA
- Experiment 22 Specific Cleavage of an Oligomeric Ribonucleotide Substrate by a Ribozyme (RNA Enzyme). App. I Properties of Common Acids and Bases
- App. II Properties of Common Buffer Compounds
- App. III pK[subscript a] Values and pH[subscript l] Values of Amino Acids
- App. IV Molecular Weights of Some Common Proteins
- App. V Common Abbreviations used in this Text
- App. VI Units of Measurement
- App. VII Table of the Elements
- App. VIII Values of t for Analysis of Statistical Confidence Limits
- App. IX Solutions to Selected Questions.