Modern experimental biochemistry /
Tallennettuna:
| Päätekijä: | |
|---|---|
| Aineistotyyppi: | Kirja |
| Kieli: | englanti |
| Julkaistu: |
San Francisco :
Benjamin Cummings,
c2000. [reprinted]
|
| Painos: | 3rd ed. |
| Aiheet: | |
| Tagit: |
Ei tageja, Lisää ensimmäinen tagi!
|
MARC
| LEADER | 00000nam a22000004a 4500 | ||
|---|---|---|---|
| 001 | 37088 | ||
| 003 | BD-DhMIS | ||
| 005 | 20241209020006.0 | ||
| 008 | 140203r2000 caua b 001 0 eng d | ||
| 010 | |a 00044528 | ||
| 015 | |a GBA0-60078 | ||
| 016 | 7 | |a 101159649 |2 DNLM | |
| 020 | |a 0805331115 (pbk.) | ||
| 020 | |a 817808239X | ||
| 035 | |a (OCoLC)ocm44493241 | ||
| 035 | |a (NNC)5805381 | ||
| 040 | |a DLC |c DLC |d C#P |d UKM |d NLM |d MUQ |d BAKER |d NNC |d OrLoB-B |d BD-DhMIS | ||
| 042 | |a pcc | ||
| 050 | 0 | 0 | |a QP519.7 |b .B68 2000 |
| 060 | 0 | 0 | |a 2003 B-458 |
| 060 | 1 | 0 | |a QU 4 |b B791m 2000 |
| 082 | 0 | 0 | |a 572/.028 |2 21 |
| 100 | 1 | |a Boyer, Rodney F. | |
| 245 | 1 | 0 | |a Modern experimental biochemistry / |c Rodney Boyer. |
| 250 | |a 3rd ed. | ||
| 260 | |a San Francisco : |b Benjamin Cummings, |c c2000. [reprinted] | ||
| 300 | |a xviii, 475 p. : |b ill. ; |c 24 cm. | ||
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 2 | 0 | |g Pt. I. |t Theory and Experimental Techniques -- |g Ch. 1. |t Introduction to the Biochemistry Laboratory -- |g A. |t Safety in the Laboratory -- |g B. |t The Laboratory Notebook -- |g C. |t Cleaning Laboratory Glassware -- |g D. |t Quantitative Transfer of Liquids -- |g E. |t Preparation and Storage of Solutions -- |g F. |t Statistical Analysis of Experimental Measurements -- |g Ch. 2. |t General Laboratory Procedures -- |g A. |t pH Measurements, Buffers, and Electrodes -- |g B. |t Dialysis and Ultrafiltration -- |g C. |t Lyophilization (Freeze-Drying) -- |g D. |t Measurement of Protein Solutions -- |g E. |t Measurement of Nucleic Acid Solutions by Spectrophotometry -- |g Ch. 3. |t Separation and Purification of Biomolecules by Chromatography -- |g A. |t Introduction to Chromatography -- |g B. |t Paper and Thin-Layer Chromatography -- |g C. |t Gas Chromatography -- |g D. |t Column Chromatography (GC) -- |g E. |t Ion-Exchange Chromatography -- |g F. |t Gel Exclusion Chromatography -- |g G. |t High-Performance Liquid Chromatography (HPLC) -- |g H. |t Affinity Chromatography and Immunoadsorption -- |g I. |t Perfusion Chromatography -- |g Ch. 4. |t Characterization of Proteins and Nucleic Acids by Electrophoresis -- |g A. |t Theory of Electrophoresis -- |g B. |t Methods of Electrophoresis -- |g C. |t Practical Aspects of Electrophoresis -- |g Ch. 5. |t Spectroscopic Analysis of Biomolecules -- |g A. |t Ultraviolet-Visible Absorption Spectrophotometry -- |g B. |t Fluorescence Spectrophotometry -- |g Ch. 6. |t Radioisotopes in Biochemical Research -- |g A. |t Origin and Properties of Radioactivity -- |g B. |t Detection and Measurement of Radioactivity -- |g C. |t Safety Rules for Handling Radioactive Materials -- |g Ch. 7. |t Centrifugation of Biomolecules -- |g A. |t Basic Principles of Centrifugation -- |g B. |t Instrumentation for Centrifugation -- |g C. |t Applications of Centrifugation -- |g Pt. II. |t Experiments -- |t Experiment 1 Using the Biochemical Literature -- |t Experiment 2 Analytical Methods for Amino Acid Separation and Identification -- |t Experiment 3 Purification and Characterization of [alpha]-Lactalbumin, a Milk Protein -- |t Experiment 4 Structural Analysis of a Dipeptide or Tripeptide -- |t Experiment 5 Protein-Ligand Interactions: Binding of Coomassie Blue Dye to Ovalbumin -- |t Experiment 6 Kinetic Analysis of an Enzyme, Tyrosinase -- |t Experiment 7 Purification and Characterization of a Lipid Extracted from Nutmeg -- |t Experiment 8 Fatty Acid Content of Triacylglycerols in Natural Oils -- |t Experiment 9 Identification of Carbohydrates by Polarimetry -- |t Experiment 10 Characterization of Hemoglobin A[subscript 1c] by Affinity Chromatography -- |t Experiment 11 Isolation, Separation and Spectrophotometric Characterization of Photosynthetic Pigments -- |t Experiment 12 Photoinduced Proton Transport Through Chloroplast Membranes -- |t Experiment 13 Isolation, Subfractionation and Enzymatic Analysis of Beef Heart Mitochondria -- |t Experiment 14 Cholesterol (Total and HDL) and Uric Acid Content of Blood Serum: Enzymes as Reagents in Clinical Chemistry -- |t Experiment 15 Vitamin C Content of Fruits, Vegetables and Other Foods -- |t Experiment 16 Activity and Thermal Stability of Acrylamide Gel-Immobilized Peroxidase: An Experiment in Biotechnology -- |t Experiment 17 Extraction of Chromosomal DNA from Bacterial Cells -- |t Experiment 18 Characterization of Nucleic Acids by Fluorescence of Bound Ethidium Bromide -- |t Experiment 19 Preparation, Isolation, and Purification of Bacterial Plasmid DNA -- |t Experiment 20 Rapid, Microscale Isolation of Plasmid DNA -- |t Experiment 21 The Action of Restriction Endonucleases on Plasmid or Viral DNA -- |t Experiment 22 Specific Cleavage of an Oligomeric Ribonucleotide Substrate by a Ribozyme (RNA Enzyme). |t App. I Properties of Common Acids and Bases -- |t App. II Properties of Common Buffer Compounds -- |t App. III pK[subscript a] Values and pH[subscript l] Values of Amino Acids -- |t App. IV Molecular Weights of Some Common Proteins -- |t App. V Common Abbreviations used in this Text -- |t App. VI Units of Measurement -- |t App. VII Table of the Elements -- |t App. VIII Values of t for Analysis of Statistical Confidence Limits -- |t App. IX Solutions to Selected Questions. |
| 541 | |e 37088 | ||
| 650 | 0 | |a Biochemistry |x Methodology. | |
| 650 | 0 | |a Biochemistry |v Laboratory manuals. | |
| 650 | 0 | |a Molecular biology |x Methodology. | |
| 650 | 0 | |a Molecular biology |v Laboratory manuals. | |
| 650 | 1 | 2 | |a Biochemistry |x methods. |
| 650 | 2 | 2 | |a Microbiological Techniques |x methods. |
| 650 | 2 | 2 | |a Molecular Biology |x methods. |
| 650 | 6 | |a Biochimie |x Méthodologie. | |
| 650 | 6 | |a Biochimie |v Manuels de laboratoire. | |
| 650 | 6 | |a Biologie moléculaire |x Méthodologie. | |
| 650 | 6 | |a Biologie moléculaire |v Manuels de laboratoire. | |
| 900 | |a AUTH |b TOC | ||
| 942 | |2 ddc |c REF |0 3 | ||
| 952 | |0 0 |1 0 |2 ddc |4 0 |6 572_028000000000000_BOY |7 0 |9 29059 |a MISTCL |b MISTCL |d 2014-02-03 |e Shahitta cosh |g 365.00 |l 2 |m 3 |o 572/.028 BOY |p 3010037088 |r 2025-01-05 00:00:00 |s 2024-12-08 |t 1 |w 2014-02-03 |y REF | ||
| 952 | |0 0 |1 0 |2 ddc |4 0 |6 572_000000000000000__028_BOY |7 0 |9 52160 |a MISTCL |b MISTCL |d 2016-03-20 |e Donation |l 1 |o 572/.028 BOY |p 3010056388 |r 2018-09-04 00:00:00 |s 2018-07-26 |t 2 |w 2016-03-20 |y REF | ||
| 999 | |c 3828 |d 3828 | ||